CoraLite® Plus 488-conjugated DVL1 Monoclonal antibody

DVL1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A2A5

Cat no : CL488-67672

Synonyms

Dishevelled 1, DSH homolog 1, DVL, DVL1

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Validation Data Gallery

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  IF Staining of MCF-7 using CL488-67672

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 DVL1 antibody (CL488-67672, Clone: 1A2A5 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67672 targets DVL1 in IF applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: DVL1 fusion protein Ag26083
• Full Name: dishevelled, dsh homolog 1 (Drosophila)
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC050454
• Gene Symbol: DVL1
• Gene ID (NCBI): 1855
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway. There are two isoforms of DVL1: 75 kDa and 73 kDa.