Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, HepG2 cells, Jurkat cells, K-562 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67739-1-Ig targets MARS in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6961 Product name: Recombinant human MARS protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 552-900 aa of BC002384 Sequence: MAKDNVPFHSLVFPCSALGAEDNYTLVSHLIATEYLNYEDGKFSKSRGVGVFGDMAQDTGIPADIWRFYLLYIRPEGQDSAFSWTDLLLKNNSELLNNLGNFINRAGMFVSKFFGGYVPEMVLTPDDQRLLAHVTLELQHYHQLLEKVRIRDALRSILTISRHGNQYIQVNEPWKRIKGSEADRQRAGTVTGLAVNIAALLSVMLQPYMPTVSATIQAQLQLPPPACSILLTNFLCTLPAGHQIGTVSPLFQKLENDQIESLRQRFGGGQAKTSPKPAVVETVTTAKPQQIQALMDEVTKQGNIVRELKAQKADKNEVAAEVAKLLDLKKQLAVAEGKPPEAPKGKKKK Predict reactive species |
| Full Name | methionyl-tRNA synthetase |
| Calculated Molecular Weight | 101 kDa |
| Observed Molecular Weight | 97-101 kDa |
| GenBank Accession Number | BC002384 |
| Gene Symbol | MARS |
| Gene ID (NCBI) | 4141 |
| RRID | AB_2918508 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P56192 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MARS antibody 67739-1-Ig | Download protocol |
| WB protocol for MARS antibody 67739-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MARS Monoclonal antibody (3E3B4), catalog number 67739-1-Ig, has a total of 2 citations. These appear in iScience and World Journal of Gastrointestinal Oncology. The cited research spans cell biology (kinectin 1 interaction with the multi-tRNA synthetase complex and ER sheet organization) and oncology (MARS1's role in hepatocellular carcinoma and its prognostic gene profile). Both studies utilized the antibody for WB, with one also employing IF.
| Species | Application | Title |
|---|---|---|
iScience Variant-specific interaction of kinectin 1 with the multi-tRNA synthetase complex regulates ER sheet organization. | ||
World J Gastrointest Oncol Methionyl-tRNA synthetase 1 participates in hepatocellular carcinoma and its regulated gene profile possesses potent prognostic ability.
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