Tested Applications
| Positive WB detected in | HCT 116 cells, HSC-T6 cells, 4T1 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67897-1-Ig targets Importin Alpha 5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12751 Product name: Recombinant human KPNA1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 19-272 aa of BC002374 Sequence: LNPDEMRRRREEEGLQLRKQKREEQLFKRRNVATAEEETEEEVMSDGGFHEAQINNMEMAPGGVITSDMIEMIFSKSPEQQLSATQKFRKLLSKEPNPPIDEVISTPGVVARFVEFLKRKENCTLQFESAWVLTNIASGNSLQTRIVIQAGAVPIFIELLSSEFEDVQEQAVWALGNIAGDSTMCRDYVLDCNILPPLLQLFSKQNRLTMTRNAVWALSNLCRGKSPPPEFAKVSPCLNVLSWLLFVSDTDVLA Predict reactive species |
| Full Name | karyopherin alpha 1 (importin alpha 5) |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60-62 kDa |
| GenBank Accession Number | BC002374 |
| Gene Symbol | Importin Alpha 5 |
| Gene ID (NCBI) | 3836 |
| RRID | AB_2918653 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P52294 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Importins (alpha and beta) are transport factors that transport proteins into the nucleus. alpha importins function by binding transcription factors at nuclear localization sequences (NLS), and associating with importin beta subunits to translocate through the nuclear pore. Six isoforms of alpha importins have been described in human. Importin alpha 5 is the predominant importin in adult CNS and is essential for differentiation and viability of neural cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Importin Alpha 5 antibody 67897-1-Ig | Download protocol |
| IHC protocol for Importin Alpha 5 antibody 67897-1-Ig | Download protocol |
| WB protocol for Importin Alpha 5 antibody 67897-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Importin Alpha 5 Monoclonal antibody (2A7G7), catalog number 67897-1-Ig, has a total of 1 citation. The citation appears in bioRxiv (2024), where the antibody was used for immunofluorescence (IF) in human samples. The associated research field is cell biology, specifically investigating how palmitoylated Importin α regulates mitotic spindle orientation through its interaction with NuMA.
| Species | Application | Title |
|---|---|---|
bioRxiv Palmitoylated Importin α Regulates Mitotic Spindle Orientation Through Interaction with NuMA |
