Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 68061-1-Ig
PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 68061-1-Ig
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 68061-1-Ig
WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells.
WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells.
IHC staining of rat brain using 68061-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68061-1-Ig (NSF antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68061-1-Ig (NSF antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of mouse brain using 68061-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
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rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:300-1:1200
Immunofluorescence (IF)-P
IF-P : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
68061-1-Ig targets NSF in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of PC-12 using 68061-1-Ig
PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 68061-1-Ig
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 68061-1-Ig
WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells.
IHC Figures
IHC staining of rat brain using 68061-1-Ig
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68061-1-Ig (NSF antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P Figures
IF Staining of mouse brain using 68061-1-Ig
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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