WB analysis using 68061-1-Ig (same clone as 68061-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
WB analysis of PC-12 using 68061-1-Ig (same clone as 68061-1-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
WB analysis of SH-SY5Y using 68061-1-Ig (same clone as 68061-1-PBS)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 68061-1-Ig (same clone as 68061-1-PBS)
WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 68061-1-Ig (same clone as 68061-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68061-1-Ig (NSF antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68061-1-Ig (NSF antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
IF Staining of mouse brain using 68061-1-Ig (same clone as 68061-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
WB analysis using 68061-1-Ig (same clone as 68061-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
WB analysis of PC-12 using 68061-1-Ig (same clone as 68061-1-PBS)
PC-12 cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
WB analysis of SH-SY5Y using 68061-1-Ig (same clone as 68061-1-PBS)
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 68061-1-Ig (NSF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 68061-1-Ig (same clone as 68061-1-PBS)
WB result of NSF antibody (68061-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSF transfected HEK-293 cells. This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
IHC Figures
IHC staining of rat brain using 68061-1-Ig (same clone as 68061-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68061-1-Ig (NSF antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
IF-P Figures
IF Staining of mouse brain using 68061-1-Ig (same clone as 68061-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using NSF antibody (68061-1-Ig, Clone: 2B6H6 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68061-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
KD/KO VALIDATED
NSF Monoclonal antibody
Catalog Number
68061-1-PBS
Citations
-
Dilutions
Applications
WB, IHC, IF-P, Indirect ELISA
Reactivity
human, mouse, rat, pig, rabbit, chicken
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
