Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, human placenta tissue |
| Positive IHC detected in | mouse brain tissue, human prostate cancer tissue, human liver cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68068-1-Ig targets Ferritin light chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20637 Product name: Recombinant human FTL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-175 aa of BC008439 Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD Predict reactive species |
| Full Name | ferritin, light polypeptide |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 18-20 kDa |
| GenBank Accession Number | BC008439 |
| Gene Symbol | Ferritin light chain |
| Gene ID (NCBI) | 2512 |
| RRID | AB_2918809 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02792 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ferritin light chain antibody 68068-1-Ig | Download protocol |
| IHC protocol for Ferritin light chain antibody 68068-1-Ig | Download protocol |
| WB protocol for Ferritin light chain antibody 68068-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Ferritin light chain Monoclonal antibody (Cat# 68068-1-Ig) has 20 citations, primarily used in WB. Journals include J Nanobiotechnology, Cell Death & Disease, International Journal of Biological Macromolecules, Journal of Ethnopharmacology, Biochemical Pharmacology, Inflammopharmacology, Journal of Neuroimmune Pharmacology, Neurobiology of Disease, Journal of Gastroenterology, Journal of Nutritional Biochemistry, BBA Molecular Basis of Disease, Toxicology and Applied Pharmacology, Journal of Biochemical and Molecular Toxicology, Frontiers in Oncology, Cell Biochemistry and Biophysics, Journal of Cardiovascular Development and Disease, and Translational Research. Research fields center on ferroptosis, iron metabolism, neuroinflammation, liver disease, and cancer.
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Functional ferritin-mimetic iron homeostasis nanoregulator to suppress ferroptosis and promote angiogenesis as a new therapeutic strategy for diabetic wound healing. | ||
Cell Death Dis Hypoxia aggravates ferroptosis in RPE cells by promoting the Fenton reaction. | ||
Int J Biol Macromol Conductive, injectable hydrogel equipped with tetramethylpyrazine regulates ferritinophagy and promotes spinal cord injury repair | ||
J Ethnopharmacol Anti-papillary thyroid carcinoma effects of dioscorea bulbifera L. through ferroptosis and the PI3K/AKT pathway based on network pharmacology and experimental validation | ||
Biochem Pharmacol Targeting AhR suppresses hepatocyte ferroptosis in MASH by regulating the Pten/Akt/β catenin axis | ||
Biochem Pharmacol DDX39B protects against sorafenib-induced ferroptosis by facilitating the splicing and cytoplasmic export of GPX4 pre-mRNA in hepatocellular carcinoma |
