Tested Applications
| Positive WB detected in | U2OS cells, HeLa cells, MCF-7 cells, pig heart tissue, A549 cells, HEK-293 cells, rabbit heart tissue, pig brain tissue, rabbit brain tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68148-1-Ig targets EEF1G in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.
| Tested Reactivity | human, pig, rabbit |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0218 Product name: Recombinant human EEF1G protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-400 aa of BC006520 Sequence: GLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWR Predict reactive species |
| Full Name | eukaryotic translation elongation factor 1 gamma |
| Calculated Molecular Weight | 50 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC006520 |
| Gene Symbol | EEF1G |
| Gene ID (NCBI) | 1937 |
| RRID | AB_2923672 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P26641 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EEF1G antibody 68148-1-Ig | Download protocol |
| WB protocol for EEF1G antibody 68148-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EEF1G Monoclonal antibody (1G7C6), catalog number 68148-1-Ig, has a total of 2 citations. These appear in Cell Reports and Food & Function. The cited research spans neurodevelopment (proximity labeling of radial glial ciliary proteins in the developing telencephalon) and neurodegeneration (β-hydroxybutyrylation of HSPE1 in Parkinson's disease models). The antibody was applied by immunofluorescence in mouse tissue for one study.
| Species | Application | Title |
|---|---|---|
Cell Rep Proximity labeling proteomics maps radial glial ciliary proteins across the developing telencephalon. | ||
Food Funct Ketogenic β-hydroxybutyrate-regulated β-hydroxybutyrylation of HSPE1 alleviates neuronal apoptosis in MPTP/MPP(+) induced Parkinson's disease models. |
