Filter: AllWBIF/ICC

WB analysis using 68148-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68148-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68148-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68148-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68148-1-Ig (EEF1G antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of MCF-7 using 68148-1-Ig
WB result of EEF1G antibody (68148-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1G transfected MCF-7 cells.
IF Staining of HeLa using 68148-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).
IF Staining of HeLa using 68148-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EEF1G antibody (68148-1-Ig, Clone: 1G7C6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Featured Product
KD/KO Validated

EEF1G Monoclonal antibody

EEF1G Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 68148-1-Ig

Clone No.1G7C6

Review Product

Host / Isotype

Mouse / IgG2b

Reactivity

human, pig, rabbit

Applications

WB, IF/ICC, ELISA

Synonyms

eEF 1B gamma, eEF-1B gamma, EF 1 gamma, EF1G, EF-1-gamma

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	U2OS cells, HeLa cells, MCF-7 cells, pig heart tissue, A549 cells, HEK-293 cells, rabbit heart tissue, pig brain tissue, rabbit brain tissue
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68148-1-Ig targets EEF1G in WB, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Reactivity	human, pig, rabbit
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag0218 Product name: Recombinant human EEF1G protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 140-400 aa of BC006520 Sequence: GLLDAYLKTRTFLVGERVTLADITVVCTLLWLYKQVLEPSFRQAFPNTNRWFLTCINQPQFRAVLGEVKLCEKMAQFDAKKFAETQPKKDTPRKEKGSREEKQKPQAERKEEKKAAAPAPEEEMDECEQALAAEPKAKDPFAHLPKSTFVLDEFKRKYSNEDTLSVALPYFWEHFDKDGWSLWYSEYRFPEELTQTFMSCNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWR Predict reactive species
Full Name	eukaryotic translation elongation factor 1 gamma
Calculated Molecular Weight	50 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC006520
Gene Symbol	EEF1G
Gene ID (NCBI)	1937
RRID	AB_2923672
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P26641
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Eukaryotic translation elongation factor 1 (EEF1G, synonym: EF1G) is a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.