Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 68170-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 68170-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68170-1-Ig (TOLLIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.
WB analysis of HepG2 using 68170-1-Ig
WB result of TOLLIP antibody (68170-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TOLLIP transfected HepG2 cells.
IHC staining of mouse colon using 68170-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse colon using 68170-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat colon using 68170-1-Ig
Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 68170-1-Ig (TOLLIP antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U2OS using 68170-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using TOLLIP antibody (68170-1-Ig, Clone: 1B3C4 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC experiment of Jurkat using 68170-1-Ig
1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human TOLLIP (68170-1-Ig, Clone:1B3C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

TOLLIP Monoclonal antibody

Name: TOLLIP Monoclonal antibody
Brand: Proteintech
SKU: 68170-1-Ig

TOLLIP Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 68170-1-Ig

Clone No.1B3C4

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

1B3C4, IL 1RAcPIP, toll interacting protein, Toll-interacting protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	pig brain tissue, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells, human peripheral blood platelets, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IHC detected in	mouse colon tissue, rat colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U2OS cells
Positive FC (Intra) detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)/ICC	IF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68170-1-Ig targets TOLLIP in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag32731 Product name: Recombinant human TOLLIP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-274 aa of BC012057 Sequence: MATTVSTQRGPVYIGELPQDFLRITPTQQQRQVQLDAQAAQQLQYGGAVGTVGRLNITVVQAKLAKNYGMTRMDPYCRLRLGYAVYETPTAHNGAKNPRWNKVIHCTVPPGVDSFYLEIFDERAFSMDDRIAWTHITIPESLRQGKVEDKWYSLSGRQGDDKEGMINLVMSYALLPAAMVMPPQPVVLMPTVYQQGVGYVPITGMPAVCSPGMVPVALPPAAVNAQPRCSEEDLKAIQDMFPNMDQEVIRSVLEAQRGNKDAAINSLLQMGEEP Predict reactive species
Full Name	toll interacting protein
Calculated Molecular Weight	30 kDa
Observed Molecular Weight	30 kDa
GenBank Accession Number	BC012057
Gene Symbol	TOLLIP
Gene ID (NCBI)	54472
RRID	AB_2923686
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9H0E2
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TOLLIP (Toll interacting protein), is an inhibitory adaptor protein. Tollip is a negative regulator of IL-1R and Toll-like receptors signalling pathway, involved in inflammatory immune response and inhibiting IRAK1 phosphorylation and kinase activity (PMID:11441107, PMID:10854325, PMID:11751856). The TOLLIP-dependent selective autophagy pathway plays a role in clearance of cytotoxic polyQ proteins aggregates (PubMed:25042851).

Protocols

Product Specific Protocols
FC protocol for TOLLIP antibody 68170-1-Ig	Download protocol
IF protocol for TOLLIP antibody 68170-1-Ig	Download protocol
IHC protocol for TOLLIP antibody 68170-1-Ig	Download protocol
WB protocol for TOLLIP antibody 68170-1-Ig	Download protocol