Tested Applications
| Positive WB detected in | LNCaP cells, Jurkat cells, rabbit heart tissue, HepG2 cells, pig heart tissue, HEK-293 cells, HeLa cells, rat heart tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68195-1-Ig targets APPL1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3334 Product name: Recombinant human APPL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 405-709 aa of BC028599 Sequence: QQRHESLRPAAGQSRPPTARTSSSGSLGSESTNLAALSLDSLVAPDTPIQFDIISPVCEDQPGQAKAFGQGGRRTNPFGESGGSTKSETEDSILHQLFIVRFLGSMEVKSDDHPDVVYETMRQILAARAIHNIFRMTESHLLVTCDCLKLIDPQTQVTRLTFPLPCVVLYATHQENKRLFGFVLRTSSGRSESNLSSVCYIFESNNEGEKICDSVGLAKQIALHAELDRRASEKQKEIERVKEKQQKELNKQKQIEKDLEEQSRLIAASSRPNQASSEGQFVVLSSSQSEESDLGEGGKKRESEA Predict reactive species |
| Full Name | adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 |
| Calculated Molecular Weight | 709 aa, 80 kDa |
| Observed Molecular Weight | 80 kDa |
| GenBank Accession Number | BC028599 |
| Gene Symbol | APPL1 |
| Gene ID (NCBI) | 26060 |
| RRID | AB_2935284 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UKG1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1 (APPL1), a binding partner of Akt2 and an important regulator of INS signaling, plays a key role in the regulation of INS secretion [PMID:22615370]. APPL1 interacts with adiponectin receptors and mediates the INS-sensitizing effects of adiponectin in muscle and endothelial cells. It also participates in nuclear signaling and transcriptional regulation, mostly by modulating the activity of various nuclear factors [PMID:22685329]. Apart from its role in endocytosis and endosomal transport, APPL1 was reported to undergo nucleocytoplasmic shuttling and participate in transcriptional regulation, e.g. by interactions with histone deacetylases (HDACs) [PMID:19686092].
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APPL1 antibody 68195-1-Ig | Download protocol |
| IF protocol for APPL1 antibody 68195-1-Ig | Download protocol |
| IHC protocol for APPL1 antibody 68195-1-Ig | Download protocol |
| WB protocol for APPL1 antibody 68195-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The APPL1 Monoclonal antibody (Cat# 68195-1-Ig) has 1 citation to date. The publication appeared in Microbiology Spectrum (IF 4.1, 2025), investigating how cholesterol-rich lipid rafts mediate endocytosis as a common pathway for respiratory syncytial virus entry into different host cells. The research falls within the fields of virology and infectious disease, specifically focusing on viral entry mechanisms and membrane biology.
| Species | Application | Title |
|---|---|---|
Microbiol Spectr Cholesterol-rich lipid rafts mediate endocytosis as a common pathway for respiratory syncytial virus entry into different host cells |
