Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells, MCF-7 cells, HeLa cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68224-1-Ig targets CA12 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7235 Product name: Recombinant human CA12 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-293 aa of BC000278 Sequence: VNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQGI Predict reactive species |
| Full Name | carbonic anhydrase XII |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 38-45 kDa |
| GenBank Accession Number | BC000278 |
| Gene Symbol | CA12 |
| Gene ID (NCBI) | 771 |
| RRID | AB_2935312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O43570 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CA12 antibody 68224-1-Ig | Download protocol |
| WB protocol for CA12 antibody 68224-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CA12 Monoclonal antibody (Cat# 68224-1-Ig) has 2 total citations. Both appear in European journals: European Journal of Medicinal Chemistry (IF 6.7) and European Journal of Pharmaceutical Sciences (IF 5.1). The research fields center on anticancer drug discovery, specifically the development of carbonic anhydrase IX and XII inhibitors—including ketoconazole-based and coumarin aminophosphonate compounds—as potential therapeutic agents. One study utilized the antibody for immunofluorescence (IF) in human samples.
| Species | Application | Title |
|---|---|---|
Eur J Med Chem Ketoconazole-carbonic anhydrase inhibitors as potential anticancer agents. | ||
Eur J Pharm Sci Selective Nanomolar Inhibitors of Carbonic Anhydrases IX and XII: Coumarin Aminophosphonates as Potential Anticancer Agents. |
