CoraLite® Plus 488-conjugated CA12 Monoclonal antibody

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  IF Staining of A549 using CL488-68224

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 CA12 antibody (CL488-68224, Clone: 3E1D8 ) at dilution of 1:1000.

CA12 Monoclonal Antibody for IF/ICC

Cat No. CL488-68224

Clone No.3E1D8

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

CA XII, CA12, Carbonate dehydratase XII, Carbonic anhydrase 12, carbonic anhydrase XII, CAXII, FLJ20151, HsT18816, Tumor antigen HOM RCC 3.1.3

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Tested Applications

• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68224 targets CA12 in IF/ICC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7235

  Product name: Recombinant human CA12 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 27-293 aa of BC000278

  Sequence: VNGSKWTYFGPDGENSWSKKYPSCGGLLQSPIDLHSDILQYDASLTPLEFQGYNLSANKQFLLTNNGHSVKLNLPSDMHIQGLQSRYSATQLHLHWGNPNDPHGSEHTVSGQHFAAELHIVHYNSDLYPDASTASNKSEGLAVLAVLIEMGSFNPSYDKIFSHLQHVKYKGQEAFVPGFNIEELLPERTAEYYRYRGSLTTPPCNPTVLWTVFRNPVQISQEQLLALETALYCTHMDDPSPREMINNFRQVQKFDERLVYTSFSQGI

• Full Name: carbonic anhydrase XII
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 38-45 kDa
• GenBank Accession Number: BC000278
• Gene Symbol: CA12
• Gene ID (NCBI): 771
• RRID: AB_3672979
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: O43570
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CA12 belongs to the alpha-carbonic anhydrase family. It reversible hydration of carbon dioxide. Overexpression of the zinc enzyme CA12 is observed in certain human cancers. The structure reveals a prototypical CA fold; however, two CA12 domains associate to form an isologous dimer, an observation that is confirmed by studies of the enzyme in solution.