Tested Applications
| Positive WB detected in | rat heart tissue, pig heart tissue, rabbit heart tissue, mouse heart tissue, chicken heart tissue |
| Positive IHC detected in | rat heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse heart tissue |
| Positive IF/ICC detected in | H9C2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunohistochemistry (IHC) | IHC : 1:4000-1:16000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68300-1-Ig targets Cardiac Troponin T in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.
| Tested Reactivity | human, mouse, rat, pig, rabbit, chicken |
| Cited Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7716 Product name: Recombinant human cTnT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-285 aa of BC002653 Sequence: MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK Predict reactive species |
| Full Name | troponin T type 2 (cardiac) |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 34-40 kDa |
| GenBank Accession Number | BC002653 |
| Gene Symbol | Cardiac Troponin T |
| Gene ID (NCBI) | 7139 |
| RRID | AB_3085051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P45379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. cTnT is commonly used as a marker of cardiomyogenic differentiation; in H9C2 rat cardiomyoblasts, its expression typically increases upon differentiation induction. Similar to H9C2, immortalized cardiomyocyte cell lines such as AC16 and HL-1 also show changes in the expression of cardiac markers (e.g., cTnT/TNNT2) upon differentiation induction-cardiac markers increase significantly in differentiated AC16 and H9C2 cells, whereas HL-1 already possesses a certain degree of sarcomeric structure-yet none of the three can fully recapitulate the transcriptomic and functional phenotypes of mature cardiomyocytes. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cardiac Troponin T antibody 68300-1-Ig | Download protocol |
| IHC protocol for Cardiac Troponin T antibody 68300-1-Ig | Download protocol |
| WB protocol for Cardiac Troponin T antibody 68300-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cardiac Troponin T Monoclonal antibody (4E2D8), catalog number 68300-1-Ig, has 30 citations. These appear in journals including Nature Genetics, Nature Communications, Circulation, Advanced Materials, ACS Nano, Cardiovascular Diabetology, Advanced Science, Journal of Molecular and Cellular Cardiology, European Journal of Pharmacology, iScience, and others. Research fields span cardiomyopathy, myocardial ischemia/reperfusion injury, ferroptosis, diabetic heart disease, sepsis-induced cardiac injury, heart failure, angiogenesis, and cardiac fibrosis, with applications primarily in IF and WB across human, mouse, rat, and rabbit species.
| Species | Application | Title |
|---|---|---|
Circulation Aberrant Phase Separation of Endothelial MAML1 Causes Congenital Heart Disease by Suppressing Notch Activity | ||
Adv Mater DNA Framework-Enabled Three Dimensional Organization of Antiarrhythmic Drugs for Radiofrequency Catheter Ablation | ||
Nat Genet Acute NIPBL depletion reveals in vivo dynamics of loop extrusion and its role in transcription activation. | ||
Nat Commun LTBP4 deficiency inhibits NLRP3 inflammasome activation in cardiomyocytes and attenuates heart failure in male mice | ||
Nat Commun Exercise-induced histone lactylation in monocyte-derived macrophages restores cardiac immune homeostasis and function in sepsis-induced cardiomyopathy. | ||
Reviews
What customers say
Both reviewers rated this antibody 5 stars. One user reported excellent performance in immunohistochemistry on human iPSC-derived organoids at a 1:500 dilution with overnight incubation. Another user confirmed high specificity in both Western blot and immunofluorescence, noting clear bands at the correct molecular weight and positive staining in paraffin-embedded chick heart tissue. Overall, the antibody is well-received for its specificity and reliable performance across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
REFIK (Verified Customer) (03-05-2026) | Used for human derived iPSCs derived organoids, works well with 1:500 dilution for overnight incubation in 1x PBST in 5% Donkey Serum
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Josh (Verified Customer) (04-24-2025) | Specific antibody, positive IF-P staining in paraffin embedded chick heart tissue and clear bands at the correct molecular weight when running a Western blot.
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