CoraLite® Plus 647-conjugated Cardiac Troponin T Monoclonal antibody

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  IF Staining of mouse heart using CL647-68300

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 647 Cardiac Troponin T antibody (CL647-68300, Clone: 4E2D8 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cardiac Troponin T Monoclonal Antibody for IF-P, ELISA

Cat No. CL647-68300

Clone No.4E2D8

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit, chicken

Applications

IF-P, ELISA

Synonyms

cTnT, TNNT2, 4E2D8, Troponin T, cardiac muscle

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Tested Applications

• Positive IF-P detected in: mouse heart tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-68300 targets Cardiac Troponin T in IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, chicken samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7716

  Product name: Recombinant human cTnT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-285 aa of BC002653

  Sequence: MSDIEEVVEEYEEEEQEEAAVEEQEEAAEEDAEAEAETEETRAEEDEEEEEAKEAEDGPMEESKPKPRSFMPNLVPPKIPDGERVDFDDIHRKRMEKDLNELQALIEAHFENRKKEEEELVSLKDRIERRRAERAEQQRIRNEREKERQNRLAEERARREEEENRRKAEDEARKKKALSNMMHFGGYIQKTERKSGKRQTEREKKKKILAERRKVLAIDHLNEDQLREKAKELWQSIYNLEAEKFDLQEKFKQQKYEINVLRNRINDNQKVSKTRGKAKVTGRWK

• Full Name: troponin T type 2 (cardiac)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC002653
• Gene Symbol: Cardiac Troponin T
• Gene ID (NCBI): 7139
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P45379
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.