CoraLite® Plus 488-conjugated Cardiac Troponin T Monoclonal antibody

Cardiac Troponin T Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

mouse, rat, rabbit, pig, chicken

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4E2D8

Cat no : CL488-68300

Synonyms

Cardiac muscle troponin T, CMH2, CMPD2, cTnT, RCM3, TNNT2, TnTC, troponin T type 2 (cardiac), Troponin T, cardiac muscle

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Validation Data Gallery

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  IF Staining of H9C2 using CL488-68300

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CoraLite® Plus 488 Cardiac Troponin T antibody (CL488-68300, Clone: 4E2D8 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: H9C2 cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68300 targets Cardiac Troponin T in IF applications and shows reactivity with mouse, rat, rabbit, pig, chicken samples.

• Tested Reactivity: mouse, rat, rabbit, pig, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cardiac Troponin T fusion protein Ag7716
• Full Name: troponin T type 2 (cardiac)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 34-40 kDa
• GenBank Accession Number: BC002653
• Gene Symbol: TNNT2
• Gene ID (NCBI): 7139
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cardiac Troponin T, also named as cTnT, TNNT2. This gene encodes the cardiac isoform of troponin T. The encoded protein is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Cardiac Troponin T can be detected as about 34-40 kDa.