Filter: AllWBIPIHCIF/ICC

WB analysis using 68417-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 68417-1-Ig (MPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.
WB analysis of rat brain using 68417-1-Ig
rat brain tissue were subjected to SDS PAGE followed by western blot with 68417-1-Ig (MPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 68417-1-Ig
WB result of MPI antibody (68417-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MPI transfected HEK-293 cells.
IP experiment of Jurkat using 68417-1-Ig
IP result of anti-MPI (IP:68417-1-Ig, 4ug; Detection:68417-1-Ig 1:2000) with Jurkat cells lysate 2280 ug.
IHC staining of mouse brain using 68417-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68417-1-Ig (MPI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 68417-1-Ig
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68417-1-Ig (MPI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of NIH/3T3 using 68417-1-Ig
Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using MPI antibody (68417-1-Ig, Clone: 3B10A6 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Featured Product
KD/KO Validated

MPI Monoclonal antibody

Name: MPI Monoclonal antibody
Brand: Proteintech
SKU: 68417-1-Ig

MPI Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 68417-1-Ig

Clone No.3B10A6

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Phosphomannose isomerase, Phosphohexomutase, Mannose-6-phosphate isomerase, mannose phosphate isomerase, Mannose 6 phosphate isomerase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, HEK-293 cells, rat brain tissue, A549 cells, LnCaP cells, Jurkat cells, K-562 cells, pig brain tissue
Positive IP detected in	Jurkat cells
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68417-1-Ig targets MPI in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity	human, mouse, rat, pig
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag5475 Product name: Recombinant human MPI protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-423 aa of BC046357 Sequence: MAAPRVFPLSCAVQQYAWGKMGSNSEVARLLASSDPLAQIAEDKPYAELWMGTHPRGDAKILDNRISQKTLSQWIAENQDSLGSKVKDTFNGNLPFLFKVLSVETPLSIQAHPNKELAEKLHLQAPQHYPDANHKPEMAIALTPFQGLCGFRPVEEIVTFLKKVPEFQFLIGDEAATHLKQTMSHDSQAVASSLQSCFSHLMKSEKKVVVEQLNLLVKRISQQAAAGNNMEDIFGELLLQLHQQYPGDIGCFAIYFLNLLTLKPGEAMFLEANVPHAYLKGDCVECMACSDNTVRAGLTPKFIDVPTLCEMLSYTPSSSKDRLFLPTRSQEDPYLSIYDPPVPDFTIMKTEVPGSVTEYKVLALDSASILLMVQGTVIASTPTTQTPIPLQRGGVLFIGANESVSLKLTEPKDLLIFRACCLL Predict reactive species
Full Name	mannose phosphate isomerase
Calculated Molecular Weight	47 kDa
Observed Molecular Weight	40-47 kDa
GenBank Accession Number	BC046357
Gene Symbol	MPI
Gene ID (NCBI)	4351
RRID	AB_3085132
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	P34949
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MPI(mannose-6-phosphate isomerase) is also named as PMI1 and belongs to the mannose-6-phosphate isomerase type 1 family. It is a zinc metalloenzyme that catalyzes the reversible isomerization of mannose-6-phosphate and fructose-6-phosphate. MPI plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. This proterin has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
IF protocol for MPI antibody 68417-1-Ig	Download protocol
IHC protocol for MPI antibody 68417-1-Ig	Download protocol
IP protocol for MPI antibody 68417-1-Ig	Download protocol
WB protocol for MPI antibody 68417-1-Ig	Download protocol