Tested Applications
| Positive WB detected in | PC-3 cells, HT-29 cells, Calyculin A treated HT-29 cells, Calyculin A treated PC-3 cells |
| Positive IF/ICC detected in | Calyculin A treated PC-3 cells |
| Positive FC (Intra) detected in | Calyculin A treated PC-3 cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
80067-1-RR targets Phospho-Beta Catenin (Ser33) in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | catenin (cadherin-associated protein), beta 1, 88kDa |
| Calculated Molecular Weight | 781 aa, 86 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC058926 |
| Gene Symbol | Beta Catenin |
| Gene ID (NCBI) | 1499 |
| ENSEMBL Gene ID | ENSG00000168036 |
| RRID | AB_2918861 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P35222 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines .
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-Beta Catenin (Ser33) antibody 80067-1-RR | Download protocol |
| WB protocol for Phospho-Beta Catenin (Ser33) antibody 80067-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Phospho-Beta Catenin (Ser33) Recombinant monoclonal antibody (Cat# 80067-1-RR) has 29 citations. These appear in journals including Nature Communications, Bioactive Materials, Cell Reports Medicine, Cancer Letters, Journal of Pathology, Molecular Medicine, and Biochemical Pharmacology. Research fields span Wnt/β-catenin signaling, oncology (colorectal, gastric, pancreatic, breast, liver, cervical, prostate, esophageal, osteosarcoma), fibrosis, osteogenesis, diabetes, and regenerative medicine. Applications are predominantly Western blot, with some IHC and IF.
| Species | Application | Title |
|---|---|---|
Bioact Mater Sustainability of functional hair follicle activity: Impact of spatially anchored multifunctional tetrahedral framework nucleic acids. | ||
Cell Rep Med Overcoming ADC resistance in advanced colorectal cancer by dual targeting of TROP2 and PERK to suppress Wnt/β-catenin signaling. | ||
Cell Mol Biol Lett The promoting roles of GLP1R and GIPR in stemness maintenance and multiple lineage-specific differentiation of PDLSCs. | ||
Cancer Lett YBX1 as a therapeutic target to suppress the LRP1-β-catenin-RRM1 axis and overcome gemcitabine resistance in pancreatic cancer | ||
Phytomedicine Exploring potential anti-gastric cancer mechanisms of Rhizoma Paridis through integrated computational and experimental analyses. |
