CoraLite®594-conjugated Alpha Tubulin Recombinant monoclonal antibody

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (orange), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, white), adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, green). Nuclei shown in red. ( Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, red), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, yellow). Nuclei shown in blue. (Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, red), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, yellow). Nuclei shown in blue. ( Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of human adipose-derived mesenchymal stem cells using CL594-80762

  IF in human adipose-derived mesenchymal stem cells undergoing adipogenesis. Sample was stained for neutral fat droplets (green), alpha-tubulin using CoraLite 594-conjugated Alpha Tubulin Recombinant Antibody (CL594-80762, white), and adiponectin using Adiponectin Mouse Monoclonal Antibody (66239-1-Ig) and Multi-rAb CoraLite Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (RGAM005, red). Nuclei shown in blue. (Image credit: Shuntaro Yamada, PhD, University of Bergen)

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  IF Staining of HepG2 using CL594-80762

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®594 Alpha Tubulin antibody (CL594-80762, Clone: 1G7 ) at dilution of 1:200.

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  FC experiment of HepG2 using CL594-80762

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated Alpha Tubulin Recombinant antibody (CL594-80762, Clone:1G7)(red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

• Featured Product
• KD/KO Validated

Alpha Tubulin Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra)

Cat No. CL594-80762

Clone No.1G7

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra)

Synonyms

TUBA1B, Tubulin alpha 1B chain, 1G7, Alpha-tubulin ubiquitous, EC:3.6.5.-

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, human adipose-derived mesenchymal stem cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

CL594-80762 targets Alpha Tubulin in WB, IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag18034

  Product name: Recombinant human Tubulin-Alpha protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-451 aa of BC009314

  Sequence: MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY

• Full Name: tubulin, alpha 1b
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC009314
• Gene Symbol: Alpha Tubulin
• Gene ID (NCBI): 10376
• RRID: AB_3673526
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Excitation Laser: Yellow-Green Laser (561 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P68363
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Neurosci Bull; OGT-Mediated O-GlcNAcylation of ATF2 Protects Against Sepsis-Associated Encephalopathy by Inhibiting Microglial Pyroptosis; Authors - Huan Yao