Tested Applications
| Positive WB detected in | A549 cells, 4T1 cells, HepG2 cells, K-562 cells, L-929 cells, RAW 264.7 cells, C6 cells, mouse spleen tissue, rat spleen tissue |
| Positive IHC detected in | human tonsillitis tissue, mouse lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse lung tissue |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81746-2-RR targets TGF Beta 1 in WB, IHC, IF/ICC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, rabbit, canine, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag13591 Product name: Recombinant human TGFB1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 14-258 aa of BC000125 Sequence: PLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGEVPPGPLPEAVLALYNSTRDRVAGESAEPEPEPEADYYAKEVTRVLMVETHNEIYDKFKQSTHSIYMFFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNNSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLSRGGEIEGFRLSAHCSCDSRDNTLQVDINGFTTGRRGDLATIHGMNRPFL Predict reactive species |
| Full Name | transforming growth factor, beta 1 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa |
| GenBank Accession Number | BC000125 |
| Gene Symbol | TGFB1 |
| Gene ID (NCBI) | 7040 |
| RRID | AB_3670503 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P01137 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TGF Beta 1 (TGF-β1) is a pleiotropic cytokine and a member of the TGF-β superfamily (including TGF-β1, TGF-β2, and TGF-β3). It is encoded by the TGFB1 gene and acts as a secreted protein involved in controlling cell growth, proliferation, differentiation, and apoptosis. TGF-β1 is produced by various cell types (e.g., platelets, lymphocytes, macrophages, epithelial cells, and inflammatory cells) and is secreted as a precursor requiring proteolytic activation to yield the mature, biologically active peptide. TGF-β1 plays crucial roles in immune regulation, wound healing, fibrosis, and tissue remodeling (including cardiac injury and fibrosis). It also influences cancer progression by promoting epithelial-to-mesenchymal transition (EMT) and metastasis in tumors.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for TGF Beta 1 antibody 81746-2-RR | Download protocol |
| IF protocol for TGF Beta 1 antibody 81746-2-RR | Download protocol |
| IHC protocol for TGF Beta 1 antibody 81746-2-RR | Download protocol |
| WB protocol for TGF Beta 1 antibody 81746-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TGF Beta 1 Recombinant monoclonal antibody (clone 230544B7), catalog number 81746-2-RR, has accumulated 136 citations. These publications span high-impact journals including Nature Communications, Science Advances, Advanced Materials, Advanced Science, Biomaterials, Journal of Experimental Medicine, and Cell Reports, among many others. The research fields covered include fibrosis (renal, pulmonary, cardiac, hepatic), wound healing, cancer biology and EMT, cardiovascular disease, ferroptosis, immune regulation, diabetic complications, tissue engineering, and aging. Cited applications include WB, IHC, IF, CoIP, and ELISA across human, mouse, rat, rabbit, canine, and chicken species.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Activation of Nerve Growth Factor signaling limits the response to lenvatinib in hepatocellular carcinoma | ||
Eur Heart J Left bundle branch vs biventricular pacing: mechanistic insights from a canine model. | ||
Bioact Mater Three-dimensional printed PCL/nHA scaffolds promote soft tissue functional fibrosis to repair chest wall defect via Piezo1/Ca(2+) signal during respiratory motion. | ||
Reviews
What customers say
This product has received one review with a perfect 5-star rating. The reviewer used it for Western Blot on macrophages at a 1:2000 dilution and reported that the antibody worked well. Overall, user feedback is positive, indicating reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sonam (Verified Customer) (10-16-2025) | AB worked well
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