Filter: AllWBIHCIF/ICCAffinity

WB analysis of HeLa using 82705-8-RR
WB result of GOLGA2/GM130 antibody (82705-8-RR; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-GOLGA2/GM130 transfected HeLa cells.
WB analysis using 82705-8-RR
Various lysates were subjected to SDS PAGE followed by western blot with 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of rat kidney using 82705-8-RR
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 82705-8-RR
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 82705-8-RR (GOLGA2/GM130 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 82705-8-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HeLa using 82705-8-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GOLGA2/GM130 antibody (82705-8-RR, Clone: 1M17 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-Phalloidin (green).
Affinity and Kinetic Characterization of 82705-8-RR
Biolayer interferometry (BLl) kinetic assays of 82705-8-RR against Human GM130/GOLGA2 were performed. The affinity constant is 0.942 nM.

Featured Product
KD/KO Validated

GOLGA2/GM130 Recombinant monoclonal antibody

GOLGA2/GM130 Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 82705-8-RR

Clone No.1M17

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GM130, GOLGA2, GM130;GOLGA2, GM130 autoantigen, 1M17

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 555

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IHC detected in	rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8400
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82705-8-RR targets GOLGA2/GM130 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1848 Product name: Recombinant human GM130;GOLGA2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-678 aa of BC014188 Sequence: LRAQLSLMAHPGEGDGLDREEEEDEEEEEEEAVAVPQPMPSIPEDLESREAMVAFFNSAVASAEEEQARLRGQLKEQRVRCRRLAHLLASAQKEPEAAAPAPGTGGDSVCGETHRALQGAMEKLQSRFMELMQEKADLKERVEELEHRCIQLSGETDTIGEYIALYQSQRAVLKERHREKEEYISRLAQDKEEMKVKLLELQELVLRLVGDRNEWHGRFLAAAQNPADEPTSGAPAPQELGAANQQGDLCEVSLAGSVEPAQGEAREGSPRDNPTAQQIMQLLREMQNPRERPGLGSNPCIPFFYRADENDEVKITVI Predict reactive species
Full Name	golgi autoantigen, golgin subfamily a, 2
Calculated Molecular Weight	111 kDa
Observed Molecular Weight	130-140 kDa
GenBank Accession Number	BC014188
Gene Symbol	GM130
Gene ID (NCBI)	2801
RRID	AB_3086513
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q08379
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GOLGA2, also known as GM130, is a 130 kDa cis-Golgi matrix protein which is one component of the detergent and salt resistant Golgi matrix. It is a peripheral membrane protein highly bound to Golgi membrane and localized mainly at the cytoplasmic face of cis-Golgi membrane. Together with its interacting partner proteins, including p115, giantin, GRASP65, and Rab GTPase, GOLGA2/GM130 is involved in the regulation of ER-to-Golgi transport and also in the maintenance of the Golgi structure. Emerging evidence suggest that the GOLGA2/GM130 has potential roles in the control of glycosylation, cell cycle progression, and higher order cell functions such as cell polarization and directed cell migration. (PMID: 20197635)

Protocols

Product Specific Protocols
IF protocol for GOLGA2/GM130 antibody 82705-8-RR	Download protocol
IHC protocol for GOLGA2/GM130 antibody 82705-8-RR	Download protocol
WB protocol for GOLGA2/GM130 antibody 82705-8-RR	Download protocol