Tested Applications
| Positive WB detected in | NCI-H1299 cells, HeLa cells, DU 145 cells, PC-3 cells, U2OS celss |
| Positive IHC detected in | human ovarian cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82742-3-RR targets BOP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag26669 Product name: Recombinant human BOP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-135 aa of BC013787 Sequence: MAGSRGAGRTAAPSVRPEKRRSEPELEPEPEPEPPLLCTSPLSHSTGSDSGVSDSEESVFSGLEDSGSDSSEDDDEGDEEGEDGALDDEGHSGIKKTTEEQVQASTPCPRTEMASARIGDEYAEDSSDEEDIRNT Predict reactive species |
| Full Name | block of proliferation 1 |
| Calculated Molecular Weight | 84 kDa |
| Observed Molecular Weight | 117 kDa |
| GenBank Accession Number | BC013787 |
| Gene Symbol | BOP1 |
| Gene ID (NCBI) | 23246 |
| RRID | AB_3086524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q14137 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Block of Proliferation 1(BOP1) protein was first isolated from a mouse, and the truncation protein may led to cell cycle arrest. Bop1 plays a role in processing of the 28S and 5.8S rRNAs (ribosomal RNAs) to regulate ribosome biogenesis. And Bop1 also act as a component of Pes1/Bop1/WDR21 (PeBoW) complex for mature 60S ribosome, and consequently acts during cell division at G1 checkpoints (PMID:18670790; PMID:26940494). What's more, abnormal expression of BOP1 was associated with liver or colorectal cancers (PMID:16804918). The molecular weight of the protein we detected was slightly higher, about 117 kd, which is also seen in the article (PMID: 30100995).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BOP1 antibody 82742-3-RR | Download protocol |
| IHC protocol for BOP1 antibody 82742-3-RR | Download protocol |
| WB protocol for BOP1 antibody 82742-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BOP1 Recombinant monoclonal antibody (3A4), catalog number 82742-3-RR, has 1 total citation. It appears in Journal of Translational Medicine (Impact Factor 9.7), published in 2026. The cited study investigates how uridine metabolism promotes lung adenocarcinoma progression by activating FBL transcription via YBX1, placing this antibody's use in the research fields of cancer biology, nucleotide metabolism, and transcriptional regulation. The application used was Western Blot in human samples.
| Species | Application | Title |
|---|---|---|
J Transl Med Uridine metabolism promotes lung adenocarcinoma progression by activating FBL transcription via YBX1. |
