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  • Western Blot (WB) analysis of various lysates using SNAI2/SLUG Recombinant antibody (82755-3-RR)

    WB analysis using 82755-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82755-3-RR (SNAI2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SNAI2/SLUG Recombinant antibody (82755-3-RR)

    IF Staining of A431 using 82755-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SNAI2 antibody (82755-3-RR, Clone: 250290A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SNAI2/SLUG Recombinant antibody

SNAI2/SLUG Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82755-3-RR
Clone No.250290A2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

SNAI2, Protein snail homolog 2, SLUG, SLUGH, SLUGH1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse spleen tissue, rat spleen tissue, rat testis tissue
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82755-3-RR targets SNAI2/SLUG in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SNAI2/SLUG fusion protein Ag2773 Predict reactive species
Full Name snail homolog 2 (Drosophila)
Calculated Molecular Weight 268 aa, 30 kDa
Observed Molecular Weight30-35 kDa
GenBank Accession NumberBC015895
Gene Symbol SLUG
Gene ID (NCBI) 6591
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43623
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNAI2 belongs to the Snail family of zinc finger transcription factors, which shares an evolutionarily conserved role in mesoderm formation in vertebrates and invertebrates. It tiggers epithelial-mesenchymal transitions and has a crucial role in developmental processes. Also it is a transcriptional reperssor in mediating RAF-induced expression of TJ protein, occludin(OCLN) and subsequent oncogenic transformation of epithelial cells.

Protocols

Product Specific Protocols
WB protocol for SNAI2/SLUG antibody 82755-3-RRDownload protocol
IF protocol for SNAI2/SLUG antibody 82755-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols