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  • Flow cytometry (FC) experiment of HUVEC cells using CTAGE5 Recombinant antibody (83017-1-RR)

    FC experiment of HUVEC using 83017-1-RR

    1x10^6 HUVEC cells were intracellularly stained with 0.25 ug Anti-Human CTAGE5 (83017-1-RR, Clone:230262E6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HepG2 cells using CTAGE5 Recombinant antibody (83017-1-RR)

    FC experiment of HepG2 using 83017-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human CTAGE5 (83017-1-RR, Clone:230262E6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CTAGE5 Recombinant antibody

CTAGE5 Uni-rAbTM Recombinant Antibody for FC (Intra), ELISA
Cat No. 83017-1-RR
Clone No.230262E6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), ELISA

Synonyms

MEA11, Cutaneous T-cell lymphoma-associated antigen 5, CTAGE family, member 5, CTAGE family member 5 ER export factor, cTAGE family member 5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive FC (Intra) detected inHUVEC cells, HepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83017-1-RR targets CTAGE5 in FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name CTAGE family, member 5
Calculated Molecular Weight 92 kDa
GenBank Accession NumberNM_005930
Gene Symbol CTAGE5
Gene ID (NCBI) 4253
RRIDAB_3670759
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96PC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241).

Protocols

Product Specific Protocols
FC protocol for CTAGE5 antibody 83017-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols