Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC35F4 antibody (83085-3-RR, Clone: 230360B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC35F4 antibody (83085-3-RR, Clone: 230360B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
FC experiment of U2OS using 83085-3-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human SLC35F4 (83085-3-RR, Clone:230360B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human SLC35F4 (83085-3-RR, Clone:230360B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
Affinity and Kinetic Characterization of 83085-3-RR
Biolayer interferometry (BLl) kinetic assays of 83085-3-RR against Human SLC35F4 were performed. The affinity constant is 0.92 nM.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC35F4 antibody (83085-3-RR, Clone: 230360B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).
FC (INTRA) Figures
FC experiment of U2OS using 83085-3-RR
1x10^6 U2OS cells were intracellularly stained with 0.25 ug Anti-Human SLC35F4 (83085-3-RR, Clone:230360B7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
AFFINITY Figures
Affinity and Kinetic Characterization of 83085-3-RR
Biolayer interferometry (BLl) kinetic assays of 83085-3-RR against Human SLC35F4 were performed. The affinity constant is 0.92 nM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
SLC35F4 Recombinant antibody
Catalog Number
83085-3-RR
Citations
-
Dilutions
IF/ICC : 1:200-1:800 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Applications
IF/ICC, FC (Intra), ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
