Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, NCI-H1299 cells, HaCaT cells, A549 cells |
| Positive IHC detected in | human intrahepatic cholangiocarcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83210-2-RR targets LAMA3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | laminin, alpha 3 |
| Calculated Molecular Weight | 367 kDa |
| Observed Molecular Weight | 260 kDa |
| GenBank Accession Number | NM_000227 |
| Gene Symbol | LAMA3 |
| Gene ID (NCBI) | 3909 |
| RRID | AB_3670895 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q16787 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Laminin subunit alpha 3 (LAMA3) expression is mediated by an epithelial enhancer via a synergistic effect of AP-1 binding sites.10 LAMA3 has been implicated in the invasion and progression of tumor cells and encodes for the α3 chain of laminin-5 (α3, β3, γ2, 332). Its dysfunction manifests as a blistering skin disease similar to junctional epidermolysis bullosa in humans. (PMID: 31557618)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LAMA3 antibody 83210-2-RR | Download protocol |
| WB protocol for LAMA3 antibody 83210-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LAMA3 Recombinant monoclonal antibody (230443C1), catalog number 83210-2-RR, has a total of 4 citations. These appear in Scientific Reports, Phytochemistry, and Biochemical and Biophysical Research Communications. The associated research fields include pleomorphic adenoma recurrence and malignant transformation, esophageal squamous cell carcinoma proliferation and invasion, extracellular matrix remodeling in cancer-associated fibroblasts, and periodontal ligament cell adhesion and proliferation.
| Species | Application | Title |
|---|---|---|
Sci Rep LAMA3 overexpression enhances proliferation, migration and invasion in esophageal squamous cell carcinoma based on bioinformatics and experimental validation
| ||
Sci Rep Single-cell transcriptomics reveals cellular evolution underlying pleomorphic adenoma recurrence and malignant transformation. | ||
Phytochemistry Ganocochlearcins A-P: Acyclic meroterpenoids from Ganoderma mushrooms targeting extracellular matrix remodeling in cancer-associated fibroblasts. | ||
Biochem Biophys Res Commun Laminin 411 is the predominant laminin isoform in mouse periodontal ligament cells and facilitates cell adhesion and proliferation via integrin α6β1. |
