LAMA3 Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 83210-2-RR (same clone as 83210-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83210-2-RR (LAMA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83210-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00186-2

  Sandwich ELISA standard curve of MP00186-2, Human LAMA3 Recombinant Matched Antibody Pair - PBS only. 83210-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY01122. 83210-2-PBS was HRP conjugated as the detection antibody. Range: 0.131-20 ng/mL.

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  Sample test of MP00186-2

  The mean LAMA3 concentration was determined to be 27.50 ng/mL in HeLa cell extract based on a 3.60 mg/mL extract load, 51.60 ng/mL in HepG2 cell extract based on a 5.00 mg/mL extract load and 21.80 ng/mL in A431 cell extract based on a 2.60 mg/mL extract load.

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  IHC staining of human intrahepatic cholangiocarcinoma using 83210-2-RR (same clone as 83210-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83210-2-RR (LAMA3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83210-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00186-2

  Cytometric bead array standard curve of MP00186-2, LAMA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83210-1-PBS. Detection antibody: 83210-2-PBS. Standard: SY01122. Range: 0.156-10 ng/mL.

LAMA3 Uni-rAb^® Recombinant Antibody for WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83210-2-PBS

Clone No.230443C1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

230443C1, BM600, E170, lama3a, Laminin subunit alpha 3

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Product Information

83210-2-PBS targets LAMA3 as part of a matched antibody pair:

MP00186-2: 83210-1-PBS capture and 83210-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: laminin, alpha 3
• Calculated Molecular Weight: 367 kDa
• Observed Molecular Weight: 260 kDa
• GenBank Accession Number: NM_000227
• Gene Symbol: LAMA3
• Gene ID (NCBI): 3909
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16787
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Laminin subunit alpha 3 (LAMA3) expression is mediated by an epithelial enhancer via a synergistic effect of AP-1 binding sites.10 LAMA3 has been implicated in the invasion and progression of tumor cells and encodes for the α3 chain of laminin-5 (α3, β3, γ2, 332). Its dysfunction manifests as a blistering skin disease similar to junctional epidermolysis bullosa in humans. (PMID: 31557618)