Filter: AllWBIHCFC (Intra)Cytometric bead arrayAffinity

WB analysis using 83398-1-RR (same clone as 83398-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83398-1-RR (MOSPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 83398-1-RR (same clone as 83398-1-PBS)
WB result of MOSPD2 antibody (83398-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MOSPD2 transfected HeLa cells. This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 83398-1-RR (same clone as 83398-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.
IHC staining of mouse placenta using 83398-1-RR (same clone as 83398-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.
FC experiment of A431 using 83398-1-RR (same clone as 83398-1-PBS)
1x10^6 A431 cells were intracellularly stained with 0.25 ug MOSPD2 Recombinant antibody (83398-1-RR, Clone:240282C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.
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1x10^6 A431 cells were intracellularly stained with 0.25 ug MOSPD2 Recombinant antibody (83398-1-RR, Clone:240282C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83398-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP00419-2
Cytometric bead array standard curve of MP00419-2, MOSPD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83398-1-PBS. Detection antibody: 83398-2-PBS. Standard: Ag22206. Range: 0.78-100 ng/mL.
Cytometric bead array standard curve of MP00419-3
Cytometric bead array standard curve of MP00419-3, MOSPD2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83398-3-PBS. Detection antibody: 83398-1-PBS. Standard: Ag22206. Range: 0.78-100 ng/mL.
Affinity and Kinetic Characterization of 83398-1-RR
Biolayer interferometry (BLl) kinetic assays of 83398-1 -RR against Human MOSPD2 were performed. The affinity constant is 0.245 nM.

Featured Product
KD/KO Validated

MOSPD2 Recombinant antibody, PBS Only (Capture/Detector)

MOSPD2 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 83398-1-PBS

Clone No.240282C11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

240282C11, Motile sperm domain-containing protein 2

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83398-1-PBS targets MOSPD2 as part of a matched antibody pair:

MP00419-2: 83398-1-PBS capture and 83398-2-PBS detection (validated in Cytometric bead array)

MP00419-3: 83398-3-PBS capture and 83398-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	MOSPD2 fusion protein Ag22206 Predict reactive species
Full Name	motile sperm domain containing 2
Calculated Molecular Weight	518 aa, 60 kDa
Observed Molecular Weight	59 kDa
GenBank Accession Number	BC030641
Gene Symbol	MOSPD2
Gene ID (NCBI)	158747
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q8NHP6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

MOSPD2 is a 518-amino acid protein containing an N-terminal CRAL/TRIO domain, a major sperm protein (MSP) domain, and a potential C-terminal transmembrane domain. MOSPD2 is a member of the vesicle-associated membrane protein-associated protein (VAP) family and one of the endoplasmic reticulum (ER) receptors that function as tether proteins to bridge ER and other organelles to build contacts(PMID: 29858488, 35389430).