MOSPD2 Recombinant antibody

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  WB analysis of HeLa using 83398-1-RR

  WB result of MOSPD2 antibody (83398-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MOSPD2 transfected HeLa cells.

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  WB analysis using 83398-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83398-1-RR (MOSPD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83398-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse placenta using 83398-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 83398-1-RR (MOSPD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of A431 using 83398-1-RR

  1x10^6 A431 cells were intracellularly stained with 0.25 ug MOSPD2 Recombinant antibody (83398-1-RR, Clone:240282C11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83398-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83398-1 -RR against Human MOSPD2 were performed. The affinity constant is 0.245 nM.

• Featured Product
• KD/KO Validated

MOSPD2 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 83398-1-RR

Clone No.240282C11

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

240282C11, Motile sperm domain-containing protein 2

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Tested Applications

• Positive WB detected in: HuH-7 cells, HeLa cells, Jurkat cells, U-937 cells, K-562 cells, RAW 264.7 cells, rat brain tissue
• Positive IHC detected in: mouse placenta tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83398-1-RR targets MOSPD2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MOSPD2 fusion protein Ag22206
• Full Name: motile sperm domain containing 2
• Calculated Molecular Weight: 518 aa, 60 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC030641
• Gene Symbol: MOSPD2
• Gene ID (NCBI): 158747
• RRID: AB_3671050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8NHP6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MOSPD2 is a 518-amino acid protein containing an N-terminal CRAL/TRIO domain, a major sperm protein (MSP) domain, and a potential C-terminal transmembrane domain. MOSPD2 is a member of the vesicle-associated membrane protein-associated protein (VAP) family and one of the endoplasmic reticulum (ER) receptors that function as tether proteins to bridge ER and other organelles to build contacts(PMID: 29858488, 35389430).