Tested Applications
| Positive WB detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83531-5-RR targets METTL9 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag7191 Product name: Recombinant human METTL9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 36-318 aa of BC000195 Sequence: MTSGPGGPAAAAGGRKENHQWYVCNREKLCESLQAVFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFSLFMSRTSINGLLGRGSMFVFSPDQFQRLLKINPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSETMIWQLQKKKYRVLGINEWQNTGFQYDVISCLNLLDRCDQPLTLLKDIRSVLEPTRGRVILALVLPFHPYVENVGGKWEKPSEILEIKGQNWEEQVNSLPEVFRKAGFVIEAFTRLPYLCEGDMYNDYYVLDDAVFVLKPV Predict reactive species |
| Full Name | methyltransferase like 9 |
| Calculated Molecular Weight | 37 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | BC000195 |
| Gene Symbol | METTL9 |
| Gene ID (NCBI) | 51108 |
| RRID | AB_3671156 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H1A3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
METTL9 is a broad-specificity methyltransferase that mediates the formation of the majority of 1-methylhistidine(1MH) present in mouse and human proteomes.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for METTL9 antibody 83531-5-RR | Download protocol |
| WB protocol for METTL9 antibody 83531-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody catalog number 83531-5-RR (Rabbit Recombinant) has 1 total citation. It appears in NPJ Precision Oncology (IF 9.9, 2025), where it was used for IHC in human samples. The research field involves hepatocellular carcinoma, specifically exploring SIX2 targeting as a sensitization strategy for sorafenib treatment through the METTL9-SLC7A11 axis.
| Species | Application | Title |
|---|---|---|
NPJ Precis Oncol Targeting SIX2 as a novel sensitization strategy of sorafenib treatment on advanced hepatocellular carcinoma through modulating METTL9-SLC7A11 axis |
