METTL9 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 83531-5-RR (same clone as 83531-5-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 83531-5-RR (METTL9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83531-5-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 83531-5-RR (same clone as 83531-5-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug METTL9 Recombinant antibody (83531-5-RR, Clone:240510A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83531-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83531-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83531-5-RR against Human METTL9 were performed. The affinity constant is below 1 pM.

METTL9 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83531-5-PBS

Clone No.240510A2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

240510A2, CGI 81, CGI81, CGI-81, DREV

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Product Information

83531-5-PBS targets METTL9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7191

  Product name: Recombinant human METTL9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 36-318 aa of BC000195

  Sequence: MTSGPGGPAAAAGGRKENHQWYVCNREKLCESLQAVFVQSYLDQGTQIFLNNSIEKSGWLFIQLYHSFVSSVFSLFMSRTSINGLLGRGSMFVFSPDQFQRLLKINPDWKTHRLLDLGAGDGEVTKIMSPHFEEIYATELSETMIWQLQKKKYRVLGINEWQNTGFQYDVISCLNLLDRCDQPLTLLKDIRSVLEPTRGRVILALVLPFHPYVENVGGKWEKPSEILEIKGQNWEEQVNSLPEVFRKAGFVIEAFTRLPYLCEGDMYNDYYVLDDAVFVLKPV

• Full Name: methyltransferase like 9
• Calculated Molecular Weight: 37 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BC000195
• Gene Symbol: METTL9
• Gene ID (NCBI): 51108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9H1A3
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

METTL9 is a broad-specificity methyltransferase that mediates the formation of the majority of 1-methylhistidine(1MH) present in mouse and human proteomes.