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  • Western Blot (WB) analysis of HepG2 cells using HIBCH Recombinant antibody (83569-1-RR)

    WB analysis of HepG2 using 83569-1-RR

    WB result of HIBCH antibody (83569-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIBCH transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using HIBCH Recombinant antibody (83569-1-RR)

    WB analysis using 83569-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83569-1-RR (HIBCH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIBCH Recombinant antibody (83569-1-RR)

    IF Staining of HepG2 using 83569-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIBCH Recombinant antibody (83569-1-RR)

    IF Staining of HepG2 using 83569-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (83569-1-RR, Clone: 240590A3 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using HIBCH Recombinant antibody (83569-1-RR)

    FC experiment of HepG2 using 83569-1-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HIBCH Recombinant antibody (83569-1-RR, Clone:240590A3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83569-1-RR

    Affinity and Kinetic Characterization of 83569-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83569-1-RR against Human HIBCH were performed. The affinity constant is 1.37 nM.

  • Featured Product
  • KD/KO Validated

HIBCH Recombinant antibody

HIBCH Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83569-1-RR
Clone No.240590A3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

HIB-CoA hydrolase, HIB CoA hydrolase, EC:3.1.2.4, 3-hydroxyisobutyryl-coenzyme A hydrolase, 3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, rat liver tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83569-1-RR targets HIBCH in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HIBCH fusion protein Ag6149 Predict reactive species
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC067822
Gene Symbol HIBCH
Gene ID (NCBI) 26275
RRIDAB_3671182
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6NVY1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).

Protocols

Product Specific Protocols
WB protocol for HIBCH antibody 83569-1-RRDownload protocol
IF protocol for HIBCH antibody 83569-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols