Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells, Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83861-1-RR targets RPE65 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag12356 Product name: Recombinant human RPE65 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 204-533 aa of BC075036 Sequence: YNIVKIPPLQADKEDPISKSEIVVQFPCSDRFKPSYVHSFGLTPNYIVFVETPVKINLFKFLSSWSLWGANYMDCFESNETMGVWLHIADKKRKKYLNNKYRTSPFNLFHHINTYEDNGFLIVDLCCWKGFEFVYNYLYLANLRENWEEVKKNARKAPQPEVRRYVLPLNIDKADTGKNLVTLPNTTATAILCSDETIWLEPEVLFSGPRQAFEFPQINYQKYCGKPYTYAYGLGLNHFVPDRLCKLNVKTKETWVWQEPDSYPSEPIFVSHPDALEEDDGVVLSVVVSPGAGQKPAYLLILNAKDLSEVARAEVEINIPVTFHGLFKKS Predict reactive species |
| Full Name | retinal pigment epithelium-specific protein 65kDa |
| Calculated Molecular Weight | 61 kDa |
| Observed Molecular Weight | 65 kDa, 61 kDa |
| GenBank Accession Number | BC075036 |
| Gene Symbol | RPE65 |
| Gene ID (NCBI) | 6121 |
| RRID | AB_3671445 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q16518 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPE65 antibody 83861-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alex (Verified Customer) (12-16-2024) | I have tested this RPE65 primary recombinant antibody used in western blotting, dilution factor 1 in 5000 as recommended in the website, used on ARPE-19 cells. I have clear bands with minimum non-specific bands at higher molecular weight, however, the clear bands correspond to ~95 kDa and not ~65 kDa.
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