Filter: AllWBSandwich ELISASample testCytometric bead arrayAffinity

WB analysis using 83887-4-RR (same clone as 83887-4-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83887-4-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 83887-4-RR (same clone as 83887-4-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 83887-4-RR (Coagulation Factor III/Tissue Factor antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83887-4-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP00859-1
Sandwich ELISA standard curve of MP00859-1, Mouse Tissue Factor Recombinant Matched Antibody Pair - PBS only. 83887-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1102. 83887-4-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.
Sample test of MP00859-1
Serum of sixteen mice was measured. The Tissue Factor concentration of detected samples was determined to be 86.6 pg/mL with a range of 63.0-105.0 pg/mL.
Sample test of MP00859-1
Urine of mice was measured. The Tissue Factor concentration of detected samples was determined to be 244.1 pg/mL.
Sample test of MP00859-1
The mean Tissue Factor concentration was determined to be 18.4 ng/mL in mouse brain tissue extract based on a 6.0 mg/mL extract load, 13.1 ng/mL in C2C12 cell extract based on a 3.6 mg/mL extract load and 2.7 ng/mL in NIH/3T3 cell extract based on a 10.6 mg/mL extract load.
Cytometric bead array standard curve of MP00859-1
Cytometric bead array standard curve of MP00859-1, MOUSE Tissue Factor Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83887-1-PBS. Detection antibody: 83887-4-PBS. Standard: Eg1102. Range: 0.313-40 ng/mL.
Affinity and Kinetic Characterization of 83887-4-RR
Biolayer interferometry (BLl) kinetic assays of 83887-4-RR against Mouse Tissue Factor were performed. The affinity constant is 0.85 nM.

Mouse Tissue Factor/CD142 Recombinant monoclonal antibody, PBS Only (Detector)

Tissue Factor/CD142 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83887-4-PBS

Clone No.240875H3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD142, Coagulation factor III, F3, Tissue factor, 240875H3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

83887-4-PBS targets Tissue Factor/CD142 as part of a matched antibody pair:

MP00859-1: 83887-1-PBS capture and 83887-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg1102 Product name: Recombinant Mouse Tissue Factor protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 29-251 aa of Sequence: AGIPEKAFNLTWISTDFKTILEWQPKPTNYTYTVQISDRSRNWKNKCFSTTDTECDLTDEIVKDVTWAYEAKVLSVPRRNSVHGDGDQLVIHGEEPPFTNAPKFLPYRDTNLGQPVIQQFEQDGRKLNVVVKDSLTLVRKNGTFLTLRQVFGKDLGYIITYRKGSSTGKKTNITNTNEFSIDVEEGVSYCFFVQAMIFSRKTNQNSPGSSTVCTEQWKSFLGE Predict reactive species
Full Name	coagulation factor III
Calculated Molecular Weight	33kDa
Observed Molecular Weight	48-53 kDa
Gene Symbol	Tissue Factor
Gene ID (NCBI)	14066
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P20352
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108). This antibody is specific for mouse Tissue Factor.