Tested Applications
| Positive WB detected in | PC-3 cells, mouse thymus tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84089-4-RR targets TCF1/TCF7 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag5792 Product name: Recombinant human TCF7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-384 aa of BC048769 Sequence: MPQLDSGGGGAGGGDDLGAPDELLAFQDEGEEQDDKSRDSAAGPERDLAELKSSLVNESEGAAGGAGIPGVPGAGAGARGEAEALGREHAAQRLFPDKLPEPLEDGLKAPECTSGMYKETVYSAFNLLMHYPPPSGAGQHPQPQPPLHKANQPPHGVPQLSLYEHFNSPHPTPAPADISQKQVHRPLQTPDLSGFYSLTSGSMGQLPHTVSWFTHPSLMLGSGVPGHPAAIPHPAIVPPSGKQELQPFDRNLKTQAESKAEKEAKKPTIKKPLNAFMLYMKEMRAKVIAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKRRSREKHQESTTGGKRNAFGTYPEKAAAPAPFLPMTVL Predict reactive species |
| Full Name | transcription factor 7 (T-cell specific, HMG-box) |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | BC048769 |
| Gene Symbol | TCF1/TCF7 |
| Gene ID (NCBI) | 6932 |
| ENSEMBL Gene ID | ENSG00000081059 |
| RRID | AB_3671655 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P36402 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TCF7, also known as TCF1, belongs to the TCF/LEF family. TCF7 is part of the Wnt signaling pathway and plays an important role in the differentiation and self-renewal of memory T cells. It has been found that TCF7 is necessary to revive T cells in response to PD-1 blockade against viral infection or cancer. TCF7 is mainly expressed in T-cells and is also detected in proliferating intestinal epithelial cells and in the basal epithelial cells of mammary gland epithelium. TCF7 has 16 isoforms with the molecular mass of 28-54 kDa (PMID: 34044317).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TCF1/TCF7 antibody 84089-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TCF1/TCF7 Recombinant monoclonal antibody (catalog number 84089-4-RR) has 1 total citation. It appears in the journal IUBMB Life, in a 2025 study titled "ZEB1 Contributes to Drug Resistance Through Canonical Wnt Signaling in Lung Cancer." The research field associated with this citation is cancer biology, specifically lung cancer and Wnt signaling pathways.
| Species | Application | Title |
|---|---|---|
IUBMB Life ZEB1 Contributes to Drug Resistance Through Canonical Wnt Signaling in Lung Cancer |
