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Mouse CD80 Recombinant monoclonal antibody, PBS Only (Detector) (84137-4-PBS)

Mouse CD80 Recombinant monoclonal antibody, PBS Only (Detector)

Name: Mouse CD80 Recombinant monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 84137-4-PBS-100UG

CD80 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84137-4-PBS

Clone No. 241371E12

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

B7 1, B7.1, Cd28l, CD80 antigen, Ly 53

Formulation: PBS Only

PBS Only

Conjugate: Unconjugated

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Filter: AllSandwich ELISASample test

Sandwich ELISA standard curve of MP01051-4
Sandwich ELISA standard curve of MP01051-4, Mouse CD80/B7-1 Recombinant Matched Antibody Pair - PBS only. 84137-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0840. 84137-4-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sample test of MP01051-4
Serum of five mice was measured. The mouse CD80/B7-1 concentration of detected samples was determined to be 1,731.45 pg/mL with a range of 1,416.7 - 2,090.4 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sample test of MP01051-4
The mean CD80/B7-1 concentration was determined to be 33,454.1 pg/mL in C2C12 cell extract based on a 3.2 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Sample test of MP01051-4
Mouse spleen tissue was cultured in RPMI supplemented with 10% fetal bovine serum, 5 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CD80/B7-1, and measured 10.44 pg/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

84137-4-PBS targets CD80 as part of a matched antibody pair:

MP01051-4: 84137-6-PBS capture and 84137-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg0840 Product name: Recombinant Mouse CD80 protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6HIS Tag: C-6HIS Domain: 38-246 aa of NM_009855.2 Sequence: VDEQLSKSVKDKVLLPCRYNSPHEDESEDRIYWQKHDKVVLSVIAGKLKVWPEYKNRTLYDNTTYSLIILGLVLSDRGTYSCVVQKKERGTYEVKHLALVKLSIKADFSTPNITESGNPSADTKRITCFASGGFPKPRFSWLENGRELPGINTTISQDPESELYTISSQLDFNTTRNHTIKCLIKYGDAHVSEDFTWEKPPEDPPDSKN Predict reactive species
Full Name	CD80 antigen
Calculated Molecular Weight	35 kDa
GenBank Accession Number	NM_009855.2
Gene Symbol	CD80
Gene ID (NCBI)	12519
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q00609-1
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.