Tested Applications
| Positive WB detected in | HeLa cells, SH-SY5Y cells, Jurkat cells |
| Positive IP detected in | NIH/3T3 cells |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84331-4-RR targets RAPGEF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag35288 Product name: Recombinant human RAPGEF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1255-1400 aa of NM_014247.2 Sequence: GSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPP Predict reactive species |
| Full Name | Rap guanine nucleotide exchange factor (GEF) 2 |
| Calculated Molecular Weight | 167kDa |
| Observed Molecular Weight | 200 kDa |
| GenBank Accession Number | NM_014247.2 |
| Gene Symbol | RAPGEF2 |
| Gene ID (NCBI) | 9693 |
| RRID | AB_3671872 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9Y4G8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RAPGEF2 antibody 84331-4-RR | Download protocol |
| IHC protocol for RAPGEF2 antibody 84331-4-RR | Download protocol |
| IP protocol for RAPGEF2 antibody 84331-4-RR | Download protocol |
| WB protocol for RAPGEF2 antibody 84331-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAPGEF2 Recombinant monoclonal antibody (clone 241637G5), catalog number 84331-4-RR, has 1 total citation. It appears in the journal Genet Med (Genetics in Medicine), a publication focused on human genetics and clinical genomics. The cited study used the antibody for Western blot (WB) in human samples, investigating rare heterozygous de novo variants in RAPGEF2 associated with a neurodevelopmental disorder. Thus, the associated research field is human genetics/neurodevelopmental disorder research.
| Species | Application | Title |
|---|---|---|
Genet Med Rare heterozygous de novo variants in RAPGEF2 are associated with a neurodevelopmental disorder. |
