RAPGEF2 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84331-4-RR (same clone as 84331-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84331-4-RR (RAPGEF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 84331-4-RR (same clone as 84331-4-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 84331-4-RR (RAPGEF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84331-4-RR (same clone as 84331-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RAPGEF2 antibody (84331-4-RR, Clone: 241637G5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84331-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84331-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84331-4-RR against Human RAPGEF2 were performed. The affinity constant is 0.19 nM.

RAPGEF2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84331-4-PBS

Clone No.241637G5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

PDZ GEF1, NRAPGEP, nRap GEP, KIAA0313, CNrasGEF

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Product Information

84331-4-PBS targets RAPGEF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag35288

  Product name: Recombinant human RAPGEF2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1255-1400 aa of NM_014247.2

  Sequence: GSHDNIQTIQHQRSWETLPFGHTHFDYSGDPAGLWASSSHMDQIMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYWGEDSEGDTGTIKRRGGKDVSIEAESSSLTSVTTEETKPVPMPAHIAVASSTTKGLIARKEGRYREPP

• Full Name: Rap guanine nucleotide exchange factor (GEF) 2
• Calculated Molecular Weight: 167kDa
• Observed Molecular Weight: 200 kDa
• GenBank Accession Number: NM_014247.2
• Gene Symbol: RAPGEF2
• Gene ID (NCBI): 9693
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9Y4G8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.