• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of L02 cells using EPPK1 Recombinant antibody (84344-2-RR)

    WB analysis of L02 using 84344-2-RR

    L02 cells were subjected to SDS PAGE followed by western blot with 84344-2-RR (EPPK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPPK1 Recombinant antibody (84344-2-RR)

    IF Staining of HeLa using 84344-2-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EPPK1 antibody (84344-2-RR, Clone: 241633B5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of MCF-7 cells using EPPK1 Recombinant antibody (84344-2-RR)

    FC experiment of MCF-7 using 84344-2-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug EPPK1 Recombinant antibody (84344-2-RR, Clone:241633B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of HeLa cells using EPPK1 Recombinant antibody (84344-2-RR)

    FC experiment of HeLa using 84344-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug EPPK1 Recombinant antibody (84344-2-RR, Clone:241633B5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84344-2-RR

    Affinity and Kinetic Characterization of 84344-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84344-2-RR against Human EPPK1 were performed. The affinity constant is 1.61 nM.

EPPK1 Recombinant antibody

EPPK1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84344-2-RR
Clone No.241633B5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

EPPK, 241633B5, EPIPL, EPIPL1, Epiplakin

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inL02 cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84344-2-RR targets EPPK1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EPPK1 fusion protein Ag35749 Predict reactive species
Full Name epiplakin 1
Calculated Molecular Weight555 kDa
Observed Molecular Weight500-600 kDa
GenBank Accession NumberNM_031308.3
Gene Symbol EPPK1
Gene ID (NCBI) 83481
RRIDAB_3671884
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP58107
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epplakin 1 (EPPK1), belonging to the plakin family, is involved in connecting intermediate filaments and regulating their reorganization in stress response. Studies have shown that EPPK1 is involved in the progression of various cancers, such as liver cancer, cervical cancer, and bladder urothelial carcinoma.

Protocols

Product Specific Protocols
WB protocol for EPPK1 antibody 84344-2-RRDownload protocol
IF protocol for EPPK1 antibody 84344-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols