Tested Applications
| Positive WB detected in | MDA-MB-231 cells, PC-3 cells, U-87 MG cells, mouse skeletal muscle tissue, mouse heart tissue |
| Positive IHC detected in | mouse skeletal muscle tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84612-3-RR targets PDP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15453 Product name: Recombinant human PPM2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC047619 Sequence: MPAPTQLFFPLIRNCELSRIYGTACYCHHKHLCCSSSYIPQSRLRYTPHPAYATFCRPKENWWQYTQGRRYASTPQKFYLTPPQVNSILKANEYSFKVPEFDGKNVSSILGFDSNQLPANAPIEDRRSAATCLQTRGMLLGVFDGHAGCACSQA Predict reactive species |
| Full Name | protein phosphatase 2C, magnesium-dependent, catalytic subunit |
| Calculated Molecular Weight | 537 aa, 61 kDa |
| Observed Molecular Weight | 55-61 kDa |
| GenBank Accession Number | BC047619 |
| Gene Symbol | PDP1 |
| Gene ID (NCBI) | 54704 |
| RRID | AB_3743608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9P0J1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Pyruvate dehydrogenase phosphatase 1 (PDP1), also known as PPM2C, is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PDP1 antibody 84612-3-RR | Download protocol |
| WB protocol for PDP1 antibody 84612-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDP1 Recombinant monoclonal antibody (242090E7), catalog number 84612-3-RR, has a total of 1 citation. It appears in Nature Metabolism (Impact Factor 27.5), published in February 2026. The cited study investigates mitochondrial Ca²⁺ efflux in controlling neuronal metabolism and long-term memory across species, placing this antibody's use in the research fields of mitochondrial biology, neuronal metabolism, and memory mechanisms. The application used was Western blotting in mouse and rat samples.
| Species | Application | Title |
|---|---|---|
Nat Metab Mitochondrial Ca(2+) efflux controls neuronal metabolism and long-term memory across species.
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