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  • Western Blot (WB) analysis of various lysates using ASXL1 Recombinant antibody (84630-1-RR)

    WB analysis using 84630-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84630-1-RR (ASXL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84630-1-RR

    Affinity and Kinetic Characterization of 84630-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84630-1-RR against Human ASXL1 were performed. The affinity constant is 39.3 nM.

ASXL1 Recombinant antibody

ASXL1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84630-1-RR
Clone No.241563A5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

241563A5, Additional sex combs-like protein 1, KIAA0978, Polycomb group protein ASXL1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells, HL-60 cells, COLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84630-1-RR targets ASXL1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ASXL1 fusion protein Ag10290 Predict reactive species
Full Name additional sex combs like 1 (Drosophila)
Calculated Molecular Weight84aa,10 kDa; 1541aa,165 kDa
Observed Molecular Weight200-220 kDa
GenBank Accession NumberBC064984
Gene Symbol ASXL1
Gene ID (NCBI) 171023
RRIDAB_3672092
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IXJ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Additional sex combs-like 1 (ASXL1) is a member of the ASXL family, which is involved in epigenetic regulation. It is one of the mammalian Asx homologs. Mutations in the ASXL1 gene are frequently found in myeloid neoplasms, breast cancers, and prostate cancers (PMID: 30013160; PMID: 22436456; PMID: 26470845). In patients with myeloid malignancies, ASXL1 mutations are mostly heterozygous frameshift or nonsense mutations that result in C-terminal truncation (PMID: 30982744; PMID: 26095772; PMID: 32683582; PMID: 29643185).

Protocols

Product Specific Protocols
WB protocol for ASXL1 antibody 84630-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols