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  • Western Blot (WB) analysis of various lysates using CAB39 Recombinant antibody (84760-4-RR)

    WB analysis using 84760-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84760-4-RR (CAB39 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of C6 cells using CAB39 Recombinant antibody (84760-4-RR)

    IF Staining of C6 using 84760-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using CAB39 antibody (84760-4-RR, Clone: 242094G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84760-4-RR

    Affinity and Kinetic Characterization of 84760-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84760-4-RR against Human CAB39 were performed. The affinity constant is 0.304 nM.

CAB39 Recombinant monoclonal antibody

CAB39 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84760-4-RR
Clone No.242094G11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

242094G11, calcium binding protein 39, CGI 66, MO25, Protein Mo25

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, U-251 cells, MCF-7 cells, L-929 cells, C2C12 cells, C6 cells
Positive IF/ICC detected inC6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84760-4-RR targets CAB39 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag36153

Product name: Recombinant human CAB39 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-51 aa of BC020570

Sequence: MPFPFGKSHKSPADIVKNLKESMAVLEKQDISDKKAEKATEEVSKNLVAMK

Predict reactive species
Full Name calcium binding protein 39
Observed Molecular Weight 39-40 kDa
GenBank Accession NumberBC020570
Gene Symbol CAB39
Gene ID (NCBI) 51719
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y376
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAB39 (Calcium-binding protein 39), also called MO25, is a component of the complex that binds and activates STK11/LKB1. It is required to stabilize the interaction between CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta) and STK11/LKB1 (PMID: 19892943).

Protocols

Product Specific Protocols
WB protocol for CAB39 antibody 84760-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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