Tested Applications
| Positive WB detected in | HepG2 cells, HEK-293 cells, HeLa cells |
| Positive ChIP-qPCR detected in | A-673 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| CHIP-QPCR | CHIP-QPCR : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84903-1-RR targets MKL1/MRTF-A in WB, ELISA, ChIP-qPCR applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag15308 Product name: Recombinant human MKL1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 392-741 aa of BC115039 Sequence: KAPAATSILHKAGEVVVAFPAARLSTGPALVAAGLAPAEVVVATVASSGVVKFGSTGSTPPVSPTPSERSLLSTGDENSTPGDTFGEMVTSPLTQLTLQASPLQILVKEEGPRAGSCCLSPGGRAELEGRDKDQMLQEKDKQIEALTRMLRQKQQLVERLKLQLEQEKRAQQPAPAPAPLGTPVKQENSFSSCQLSQQPLGPAHPFNPSLAAPATNHIDPCAVAPGPPSVVVKQEALQPEPEPVPAPQLLLGPQGPGLIKGVAPPTLITDSTGTHLVLTVTNKNADSPGLSSGSPQQPSSQPGSPAPAPSAQMDLEHPLQPLFGTPTSLLKKEPPGYEEAMSQQPKQQEN Predict reactive species |
| Full Name | megakaryoblastic leukemia (translocation) 1 |
| Calculated Molecular Weight | 931 aa, 99 kDa |
| Observed Molecular Weight | 145-160 kDa |
| GenBank Accession Number | BC115039 |
| Gene Symbol | MKL1 |
| Gene ID (NCBI) | 57591 |
| RRID | AB_3743828 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q969V6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Megakaryocytic leukemia 1 (MKL1), also known as myocardin-related transcription factor A (MRTF-A), is a transcriptional regulator involved in a wide range of pathophysiological processes in the cardiovascular system. MKL1 was initially identified as a co-factor for serum response factor (SRF) to regulate the transcription of contractile genes. Forced expression of MKL1 in non-muscle cells leads to robust up-regulation of contractile genes (PMID: 36587486, PMID: 37199168, PMID: 39222836). MKL1 is ubiquitously expressed and has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart, and brain. The molecular weight of MKL1 observed is consistent with what has been described in the literature (PMID: 34944031).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MKL1/MRTF-A antibody 84903-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MKL1/MRTF-A Recombinant monoclonal antibody (242021B4), catalog number 84903-1-RR, has 1 citation to date. The citation appears in Life Sciences (2026), where the antibody was used to study MKL1's role in promoting intestinal epithelial repair by regulating proliferation, migration, and adhesion. The associated research field is gastrointestinal biology and epithelial cell repair mechanisms.
| Species | Application | Title |
|---|---|---|
Life Sci MKL1 promotes intestinal epithelial repair by regulating proliferation, migration, and adhesion. |
