Filter: AllWBSandwich ELISASample testIF/ICCCytometric bead array

WB analysis using 84919-1-RR (same clone as 84919-1-PBS)
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84919-1-PBS in a different storage buffer formulation.
WB analysis using 84919-1-RR (same clone as 84919-1-PBS)
J774A.1 cells were subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84919-1-PBS in a different storage buffer formulation.
WB analysis of mouse spleen using 84919-1-RR (same clone as 84919-1-PBS)
mouse spleen tissue was subjected to SDS PAGE followed by western blot with 84919-1-RR (M-CSF R/CD115 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84919-1-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP01676-2
Sandwich ELISA standard curve of MP01676-2, Mouse CSF1R/CD115 Recombinant Matched Antibody Pair - PBS only. 84919-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1816. 84919-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.
Sample test of MP01676-2
Serum of eight mice was measured. The CSF1R/CD115 concentration of detected samples was determined to be 2.9 ug/mL with a range of 2.3-3.7 ug/mL.
Sample test of MP01676-2
RAW 264.7were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for mouse CSF1R/CD115, and measured 1,189.8 pg/mL.
Sample test of MP01676-2
The mean CSF1R/CD115 concentration was determined to be 760.8 ng/mL in RAW 264.7 cell extract based on a 7.5 mg/mL extract load.
IF Staining of RAW 264.7 using 84919-1-RR (same clone as 84919-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CSF1R/CD115 antibody (84919-1-RR, Clone: 242213B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84919-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP01676-2
Cytometric bead array standard curve of MP01676-2, MOUSE CSF1R/CD115 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84919-1-PBS. Detection antibody: 84919-2-PBS. Standard: Eg1816. Range: 0.625-80 ng/mL.

Mouse CSF1R/CD115 Recombinant antibody, PBS Only (Capture)

CSF1R/CD115 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84919-1-PBS

Clone No.242213B1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Csf1r, Csfmr, CSF-1 receptor, CSF 1R, CD115

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84919-1-PBS targets CSF1R/CD115 as part of a matched antibody pair:

MP01676-2: 84919-1-PBS capture and 84919-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Recombinant protein Predict reactive species
Full Name	colony stimulating factor 1 receptor
Calculated Molecular Weight	109 kDa
Observed Molecular Weight	110-170 kDa
GenBank Accession Number	NM_001037859.2
Gene Symbol	CSF1R/CD115
Gene ID (NCBI)	12978
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P09581
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).