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  • Immunofluorescence (IF) / fluorescent staining of A431 cells using NCOA5 Recombinant antibody (85004-1-RR)

    IF Staining of A431 using 85004-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using NCOA5 antibody (85004-1-RR, Clone: 241969G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of HEK-293 cells using NCOA5 Recombinant antibody (85004-1-RR)

    FC experiment of HEK-293 using 85004-1-RR

    1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug NCOA5 Recombinant antibody (85004-1-RR, Clone:241969G3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

NCOA5 Recombinant antibody

NCOA5 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85004-1-RR
Clone No.241969G3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

Nuclear receptor coactivator 5, NCoA-5, KIAA1637, Coactivator independent of AF-2, CIA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85004-1-RR targets NCOA5 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen NCOA5 fusion protein Ag14418 Predict reactive species
Full Name nuclear receptor coactivator 5
Calculated Molecular Weight 579 aa, 66 kDa
GenBank Accession NumberBC056872
Gene Symbol NCOA5
Gene ID (NCBI) 57727
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9HCD5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear receptor coactivator 5 (NCOA5), also known as a coactivator independent of AF-2 (CIA), possesses both repressor and activator functions (PMID: 11113208). It can interact with Estrogen Receptor (ER) alpha and ER beta in a ligand-dependent manner. NCOA5 has been shown to regulate the ER alpha-mediated transcription and the c-Myc gene expression (PMID: 11113208; 15073177). Recently identified as a conserved component of pluripotent stem cells, mammalian NCOA5 may contribute to maintaining the pluripotent stem cell population (PMID: 24268775).

Protocols

Product Specific Protocols
IF protocol for NCOA5 antibody 85004-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols