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  • Western Blot (WB) analysis of Ramos cells using TCL1A Recombinant antibody (85129-5-RR)

    WB analysis of Ramos using 85129-5-RR

    Ramos cells were subjected to SDS PAGE followed by western blot with 85129-5-RR (TCL1A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using TCL1A Recombinant antibody (85129-5-RR)

    IHC staining of human tonsillitis using 85129-5-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 85129-5-RR (TCL1A antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using TCL1A Recombinant antibody (85129-5-RR)

    IF Staining of Ramos using 85129-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using TCL1A antibody (85129-5-RR, Clone: 242623A9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of Ramos cells using TCL1A Recombinant antibody (85129-5-RR)

    FC experiment of Ramos using 85129-5-RR

    1x10^6 Ramos cells were intracellularly stained with 0.25 ug TCL1A Recombinant antibody (85129-5-RR, Clone:242623A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85129-5-RR

    Affinity and Kinetic Characterization of 85129-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85129-5-RR against Human TCL1A were performed. The affinity constant is 0.185 nM.

TCL1A Recombinant antibody

TCL1A Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 85129-5-RR
Clone No.242623A9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

242623A9, Oncogene TCL-1, T-cell leukemia/lymphoma protein 1A, TCL1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inRamos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRamos cells
Positive FC (Intra) detected inRamos cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85129-5-RR targets TCL1A in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TCL1A fusion protein Ag0786 Predict reactive species
Full Name T-cell leukemia/lymphoma 1A
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC005831
Gene Symbol TCL1A
Gene ID (NCBI) 8115
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP56279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCL1A, also named as TCL1 and p14 TCL1, enhances cell proliferation, stabilizes mitochondrial membrane potential and promotes cell survival. TCL1A immunodetection is an independent marker of adverse outcome that could be used in routine settings for the management of DLCL patients. TCL1A expression is correlated with shorter time to treatment in chronic lymphocytic leukemia cases and shorter lymphoma-specific survival in mantle cell lymphoma series. It is a potential therapeutic target.

Protocols

Product Specific Protocols
WB protocol for TCL1A antibody 85129-5-RRDownload protocol
IHC protocol for TCL1A antibody 85129-5-RRDownload protocol
IF protocol for TCL1A antibody 85129-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols