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  • Western Blot (WB) analysis of various lysates using ILK Recombinant antibody (85428-5-RR)

    WB analysis using 85428-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85428-5-RR (ILK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig colon tissue using ILK Recombinant antibody (85428-5-RR)

    WB analysis of pig colon using 85428-5-RR

    pig colon tissue were subjected to SDS PAGE followed by western blot with 85428-5-RR (ILK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ILK Recombinant antibody (85428-5-RR)

    IF Staining of HeLa using 85428-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ILK antibody (85428-5-RR, Clone: 242982A5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85428-5-RR

    Affinity and Kinetic Characterization of 85428-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85428-5-RR against Human ILK were performed. The affinity constant is below 1 pM.

ILK Recombinant antibody

ILK Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85428-5-RR
Clone No.242982A5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IF/ICC, ELISA

Synonyms

59 kDa serine/threonine-protein kinase, Beta-integrin-linked kinase, EC:2.7.11.1, ILK 1, ILK 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inK-562 cells, pig colon tissue, HeLa cells, HEK-293 cells, mouse brain tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85428-5-RR targets ILK in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen ILK fusion protein Ag3570 Predict reactive species
Full Name integrin-linked kinase
Calculated Molecular Weight 451 aa, 59 kDa
Observed Molecular Weight51 kDa
GenBank Accession NumberBC001554
Gene Symbol ILK
Gene ID (NCBI) 3611
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13418
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ILK(Integrin-linked protein kinase) is also named as p59, PAT-4, DKFZp686F1765, ILK-1, ILK-2, ILK1, ILK2, p59ILK and belongs to the TKL Ser/Thr protein kinase family. This protein localizes to multiple sites, including the cytoplasm, and imports into the nucleus through sequences in its N-terminus, via active transport mechanisms that involve nuclear pore complexes(PMID: 18635968). ILK performs crucial roles in the control of intestinal cell and crypt-villus axis homeostasis-especially with regard to basement membrane fibronectin deposition-as well as cell proliferation, spreading, and migration(PMID:19885839).

Protocols

Product Specific Protocols
WB protocol for ILK antibody 85428-5-RRDownload protocol
IF protocol for ILK antibody 85428-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols