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  • Western Blot (WB) analysis of various lysates using SKP2 Recombinant antibody (85670-4-RR)

    WB analysis using 85670-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85670-4-RR (SKP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SKP2 Recombinant antibody (85670-4-RR)

    IF Staining of HepG2 using 85670-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SKP2 antibody (85670-4-RR, Clone: 243080H6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SKP2 Recombinant antibody

SKP2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85670-4-RR
Clone No.243080H6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Cyclin-A/CDK2-associated protein p45, F box protein Skp2, F box/LRR repeat protein 1, FBL1, F-box protein Skp2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85670-4-RR targets SKP2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SKP2 fusion protein Ag6826 Predict reactive species
Full Name S-phase kinase-associated protein 2 (p45)
Calculated Molecular Weight 48 kDa
Observed Molecular Weight48 kDa, 46 kDa
GenBank Accession NumberBC001441
Gene Symbol SKP2
Gene ID (NCBI) 6502
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13309
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).

Protocols

Product Specific Protocols
WB protocol for SKP2 antibody 85670-4-RRDownload protocol
IF protocol for SKP2 antibody 85670-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols