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  • Western Blot (WB) analysis of various lysates using RBBP6 Recombinant antibody (85884-5-RR)

    WB analysis using 85884-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85884-5-RR (RBBP6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RBBP6 Recombinant antibody (85884-5-RR)

    IF Staining of MCF-7 using 85884-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using RBBP6 antibody (85884-5-RR, Clone: 250142C1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RBBP6 Recombinant antibody

RBBP6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85884-5-RR
Clone No.250142C1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

Synonyms

E3 ubiquitin-protein ligase RBBP6, EC:2.3.2.27, MY038, P2P R, P2PR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inEC109 cells, NIH/3T3 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85884-5-RR targets RBBP6 in applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RBBP6 fusion protein Ag2484 Predict reactive species
Full Name retinoblastoma binding protein 6
Calculated Molecular Weight 1792 aa, 200 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberBC029352
Gene Symbol RBBP6
Gene ID (NCBI) 5930
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z6E9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBBP6 (retinoblastoma binding protein 6) is a multifunctional protein that interacts with both p53 and pRb and has been implicated in mRNA processing. It has also been identified as a putative E3 ubiquitin ligase due to the presence of a RING finger domain [PMID:18851979]. RBBP6 contains an N-terminal ubiquitin-like domain and a cysteine-rich RING finger-like domain, through which it promotes the ubiquitination of p53 by Hdm2 in an E4-like manner [PMID:17470788]. It is implicated in a diverse set of cellular functions, including mRNA metabolism, regulation of the cell cycle, tumorigenesis, and development [PMID:12938151,17470788].

Protocols

Product Specific Protocols
WB protocol for RBBP6 antibody 85884-5-RRDownload protocol
IF protocol for RBBP6 antibody 85884-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols