Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | C6 cells, U-251 cells |
| Positive FC (Intra) detected in | U251 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.2 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85930-1-RR targets FOXG1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag17169 Product name: Recombinant human FOXG1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 190-489 aa of BC035020 Sequence: IMMAIRQSPEKRLTLNGIYEFIMKNFPYYRENKQGWQNSIRHNLSLNKCFVKVPRHYDDPGKGNYWMLDPSSDDVFIGGTTGKLRRRSTTSRAKLAFKRGARLTSTGLTFMDRAGSLYWPMSPFLSLHHPRASSTLSYNGTTSAYPSHPMPYSSVLTQNSLGNNHSFSTANGLSVDRLVNGEIPYATHHLTAAALAASVPCGLSVPCSGTYSLNPCSVNLLAGQTSYFFPHVPHPSMTSQSSTSMSARAASSSTSPQAPSTLPCESLRPSLPSFTTGLSGGLSDYFTHQNQGSSSNPLIH Predict reactive species |
| Full Name | forkhead box G1 |
| Calculated Molecular Weight | 489 aa, 52 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC035020 |
| Gene Symbol | FOXG1 |
| Gene ID (NCBI) | 2290 |
| RRID | AB_3744481 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P55316 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Forkhead Box G1 (FOXG1) is a member of the Forkhead family of genes with non-redundant roles in brain development, where alteration of this gene's expression significantly affects the formation and function of the mammalian cerebral cortex. FOXG1 haploinsufficiency in humans is associated with prominent differences in brain size and impaired intellectual development noticeable in early childhood, while homozygous mutations are typically fatal.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FOXG1 antibody 85930-1-RR | Download protocol |
| IF protocol for FOXG1 antibody 85930-1-RR | Download protocol |
| IHC protocol for FOXG1 antibody 85930-1-RR | Download protocol |
| WB protocol for FOXG1 antibody 85930-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FOXG1 Recombinant monoclonal antibody (250144E8), catalog number 85930-1-RR, has a total of 1 citation. The cited publication appeared in Gynecological Endocrinology and investigated the role of hsa-piR-775 in primary ovarian insufficiency through FOXG1 modulation. The research field is reproductive endocrinology, specifically focusing on ovarian function and FOXG1-mediated gene regulation in human samples using WB and IF applications.
| Species | Application | Title |
|---|---|---|
Gynecol Endocrinol Deciphering the role of hsa-piR-775 in primary ovarian insufficiency through FOXG1 modulation. |
