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  • Western Blot (WB) analysis of rat brain tissue using PUM1 Recombinant antibody (86129-1-RR)

    WB analysis of rat brain using 86129-1-RR

    rat brain tissue were subjected to SDS PAGE followed by western blot with 86129-1-RR (PUM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PUM1 Recombinant antibody (86129-1-RR)

    WB analysis using 86129-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86129-1-RR (PUM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PUM1 Recombinant antibody (86129-1-RR)

    IF Staining of HeLa using 86129-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PUM1 antibody (86129-1-RR, Clone: 250792G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PUM1 Recombinant antibody

PUM1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86129-1-RR
Clone No.250792G4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

HsPUM, KIAA0099, PUMH, PUMH1, Pumilio 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inMCF-7 cells, rat brain tissue, A549 cells, K-562 cells, HEK-293 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86129-1-RR targets PUM1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PUM1 fusion protein Ag24651 Predict reactive species
Full Name pumilio homolog 1 (Drosophila)
Calculated Molecular Weight 1186 aa, 126 kDa
Observed Molecular Weight140 kDa
GenBank Accession NumberBC013398
Gene Symbol PUM1
Gene ID (NCBI) 9698
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14671
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PUM1, also named as Pumilio homolog 1, is a 1186 amino acid protein, which is expressed in brain, heart, kidney, muscle, intestine and stomach. PUM1 as sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. PUM1 binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). The calculated molecular weight of PUM1 is 126 kDa, but the modified PUM1 is about 130-140 kDa.

Protocols

Product Specific Protocols
WB protocol for PUM1 antibody 86129-1-RRDownload protocol
IF protocol for PUM1 antibody 86129-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols