Tested Applications
| Positive WB detected in | A-204 cells, C2C12 cells, mouse heart tissue, rat heart tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86192-3-RR targets Myogenin in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag25081 Product name: Recombinant human Myogenin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 138-224 aa of BC053899 Sequence: SLNQEERDLRYRGGGGPQPGVPSECSSHSASCSPEWGSALEFSANPGDHLLTADPTDAHNLHSLTSIVDSITVEDVSVAFPDETMPN Predict reactive species |
| Full Name | myogenin (myogenic factor 4) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC053899 |
| Gene Symbol | Myogenin |
| Gene ID (NCBI) | 4656 |
| RRID | AB_3744701 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P15173 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MYOG (encoded Myogenin protein) is a muscle-specific transcription factor that belongs to a family of basic helix-loop-helix transcription factors. It plays a role in muscle differentiation, cell cycle exit, and muscle atrophy(PMID: 30315160). Diseases associated with MYOG include Embryonal Rhabdomyosarcoma and Gallbladder Sarcoma.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Myogenin antibody 86192-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Myogenin Recombinant monoclonal antibody (250187G11), catalog number 86192-3-RR, has a total of 1 citation. The citation appears in Cell Signal (2026), studying Epimedin B's amelioration of benzo[a]pyrene-induced C2C12 myotube atrophy through the p53 signaling pathway. The research field encompasses muscle atrophy and signaling pathway biology, with the antibody applied in Western Blot (WB) on mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Signal Epimedin B ameliorates benzo[a]pyrene -induced C2C12 myotube atrophy through p53 signaling pathway. |
